Scientist accused of playing God after creating artificial life by making designer microbe from scratch - but could it wipe out humanity?
By Fiona Macrae
Last updated at 3:33 PM on 3rd June 2010
Scientists today lined up to air their fears over a genome pioneer's claims that he has created artificial life in the laboratory.
In a world first, which has alarmed many, maverick biologist and billionaire entrepreneur Craig Venter, built a synthetic cell from scratch.
The creation of the new life form, which has been nicknamed 'Synthia', paves the way for customised bugs that could revolutionise healthcare and fuel production, according to its maker.
But there are fears that the research, detailed in the journal Science, could be abused to create the ultimate biological weapon, or that one mistake in a lab could lead to millions being wiped out by a plague, in scenes reminiscent of the Will Smith film I Am Legend.
While some hailed the research as 'a defining moment in the history of biology', others attacked it as 'a shot in the dark', with 'unparalleled risks'. The team involved have been accused of 'playing God' and tampering 'with the essence of life'.
Dr Venter created the lifeform by synthesising a DNA code and injecting it into a single bacteria cell. The cell containing the man-made DNA then grew and divided, creating a hitherto unseen lifeform.
Kenneth Oye, a social scientist at the Massachusetts Institute of Technology in the U.S., said: 'Right now, we are shooting in the dark as to what the long-term benefits and long-term risks will be.'
Pat Mooney, of the ETC group, a technology watchdog with a special interest in synthetic biology, said: 'This is a Pandora's box moment - like the splitting of the atom or the cloning of Dolly the sheep, we will all have to deal with the fall-out from this alarming experiment.'
Dr David King, of the Human Genetics Alert watchdog, said: 'What is really dangerous is these scientists' ambitions for total and unrestrained control over nature, which many people describe as 'playing God'.
'Scientists' understanding of biology falls far short of their technical capabilities. We have learned to our cost the risks that gap brings, for the environment, animal welfare and human health.'
Professor Julian Savulescu, an Oxford University ethicist, said: 'Venter is creaking open the most profound door in humanity's history, potentially peeking into its destiny.
'He is not merely copying life artificially or modifying it by genetic engineering. He is going towards the role of God: Creating artificial life that could never have existed.'
He said the creation of the first designer bug was a step towards 'the creation of living beings with capacities and a nature that could never have naturally evolved'. The risks were 'unparalleled',' he added.
And he warned: 'This could be used in the future to make the most powerful bioweapons imaginable. The challenge is to eat the fruit without the worm.'
Dr Venter, who was instrumental in sequencing the human genome, had previously succeeded in transplanting one bug's genome - its entire cache of DNA - into another bacterium, effectively changing its species.
He has taken this one step further, transplanting not a natural genome but a man-made one. To do this, he read the DNA of Mycoplasma mycoides, a bug that infects goats, and recreated it piece by piece.
The fragments were then 'stitched together' and inserted into a bacterium from a different species.
There, it sprang to life, allowing the bug to grow and multiply, producing generations that were entirely artificial.
The transferred DNA contained around 850 genes - a fraction of the 20,000 or so contained in a human's genetic blueprint.
In future, bacterial 'factories' could be set up to manufacture artificial organisms designed for specific tasks such as medicines or producing clean biofuels.
The technology could also be harnessed to create environmentally friendly bugs capable of mopping up carbon dioxide or toxic waste.
Dr Venter, a 63-year-old Vietnam War veteran known for his showman tendencies, said last night: 'We are entering a new era where we're limited mostly by our imaginations.'
But the breakthrough, which took 15 years and £27.7million to achieve, opens an ethical Pandora's box. Ethicists said he is 'creaking open the most profound door in humanity's history' - with unparalleled risks.
Dr Venter, whose team of 20 scientists includes a Nobel laureate, likens the process to booting-up a computer.
Like a program without a hard drive, the DNA doesn't do anything by itself. But, when the software is loaded into the computer - in this case the second bacterium - amazing things are possible, he said.
Now that the scientist, whose J Craig Venter Institute has labs in California and Maryland, has proved the concept, the path is open for him to alter the 'recipe' to create any sort of organism he chooses.
At the top of his wishlist are bugs capable of producing clean biofuels and of sucking carbon dioxide out of the atmosphere. Other possibilities include designer microbes that can mop up oil slicks or generate huge quantities of drugs, including the flu vaccine.
Any such organisms would be deliberately 'crippled' so that they cannot survive outside the lab, he claimed.
Brushing aside the ethical concerns of his work, Dr Venter wrote in his autobiography that it would allow 'a new creature to enter the world'.
'We have often been asked if this will be a step too far,' he said. 'I always reply that - so far at least - we are only reconstructing a diminished version of what is out there in nature.'
Last night, he claimed the breakthrough had changed his views on the definition of life. 'We have ended up with the first synthetic cell powered and controlled by a synthetic chromosome and made from four bottles of chemicals,' he said.
'It is pretty stunning when you just replace the DNA software in a cell and the cell instantly starts reading that new software and starts making a whole new set of proteins, and within a short while all the characteristics of the first species disappear and a new species emerges.
'That's a pretty important change in how we approach and think about life.'
The process was carried out on one of the simplest types of bacteria, under strict ethical guidelines. The research team insist that they cannot think of a day when the technology could be used to create animals or people from scratch.
Has he created a monster?
By Michael Hanlon, Science Editor
Now it is a scientific fact. Yesterday's announcement of the birth of a 'synthetic cell' - made by injecting a bacterium shell with genetic material created from scratch by scientists - raises many questions.
These range from the mundanely practical - how will this be useful? - to the profoundly philosophical - will we have to redefine what life is?
Depending on your viewpoint, it is either a powerful testament to human ingenuity or a terrible example of hubris - and the first step on a very dangerous road.
To understand what this development means, we need to discover who the team behind this innovation is.
It is led by Craig Venter, the world's greatest scientific provocateur, a 63-year-old Utah-born genius, a Vietnam veteran, billionaire, yachtsman, and an explorer. Above all he is a showman.
A master of self-publicity, he does not do things by halves; he led the private team which competed with scores of publicly funded scientists in the U.S. and UK to 'crack' the human genome by sequencing our DNA.
His rapid, innovative approach led to the possibility he would beat the scientific establishment.
So, to save face all round, the human genome was presented as a joint achievement. At around the same time, he began talking about making an artificial lifeform in the lab.
Not a Frankenstein's monster, or even a mouse, but a bacterium, one of the simplest living organisms. His blueprint was to be an unassuming and harmless little germ with only 485 genes (humans have around 25,000).
Venter talks grandly of a supercharged biotech revolution, with synthetic bacteria designed to produce biofuels, to mine precious metals from rocks and industrial waste, to digest oil slicks and render toxic spills harmless.
Scientists could even create bacteria which can produce novel drugs and vaccines, or organisms engineered to live on Mars and other planets.
The potential is huge - but so are the dangers. An artificial species, created in the lab, might not 'obey the rules' of the natural world - after all, every living being on Earth has evolved over three billion years, when a myriad of competing species have had to share the same increasingly crowded environment.
It is possible to imagine a synthetic microbe going on the rampage, perhaps wiping out all the world's crop plants or even humanity itself.
Synthetic biology also challenges our most cherished notions of what life itself actually is. Non-scientists might not realise that we have, as yet, no proper definition of life.
A diamond is not alive; a baboon clearly is. But what about a virus? Viruses, which are even simpler than bacteria, have a genetic code written in DNA (or its cousin RNA).
The stuff viruses are made from is the stuff of life - protein coats and so on - yet they cannot reproduce independently.
Like diamonds, they can be grown into crystals - and you certainly cannot crystallise baboons. Most biologists say viruses are not alive, and that true biology begins with bacteria.
So is Synthia, Venter's tentative name for his new critter, alive? It is certainly not the result of Darwinian evolution, one of the (many) definitions of life. It is more 'alive' than any virus but it is the product of Man, not of evolution. Its genetic code is simple enough to be stored on a computer (but then again, so is ours).
Whatever the answer to this fundamental question, Venter's breakthrough is certainly the final rebuttal to the old notion of a vital spark - a mysterious essence that divides the quick from the dead. If you can carry around a genome on a computer memory stick and make a cell using a few simple chemicals, then the old idea of 'vitalism' is truly dead.
Of course, this is early days. It is not yet clear if Venter can negotiate the final step - creating a whole cell from scratch, using no bits of existing living organisms at all
His bacterium is likely to be weak and feeble; we are a long way from synthetic super-plagues, and even further from an artificial animal or plant. But it is hard to escape the feeling that a boundary has been crossed. The problem is, it is far from clear where we go from here.
'WATERMARKING' DNA
This dramatic development naturally raises fears of the dangers these organisms pose. So one idea, which has been followed through by Venter and his team, is to 'watermark' them.
By weaving these hidden codes in it enables scientists to trace the organisms to their laboratories and prove the recipient bacteria contained the synthetic genome.
Researchers used the 'alphabet' of genes and proteins to spell out messages.
The team created a code that spells out the 26 letters of the alphabet, the numbers 0to 9 and several punctuation marks. They then wrote a message which reveals the code. A second missive was a string of 'letters' corresponding to the names of 46 people involved in the project. A third gave an e-mail address where people can write once they crack the code and the fourth listed three philosophical quotes.
WHO IS CRAIG VENTER?
Craig Venter is a controversial biologist and entrepreneur who led the effort by the private sector to sequence the human genome.
He was vilified by the scientific community for turning the project into a competitive race but his efforts did mean that the human genome was mapped three years earlier than expected.
Born in 1946, Dr Venter was an average scholar with a keen interest in surfing.
It was while serving in Vietnam and tending to wounded comrades that he was inspired to become a doctor.
During his medical training he excelled in research and was quick to realise the importance of decoding genes. In 1992 he set up the private Institute for Genomic Research. Then a mere three years later he stunned the scientific establishment by revealing the first complete genome of a free-living organism that causes childhood ear infections and meningitis.
In 2005 he founded the private company Synthetic Genomics, with the aim of engineering new life forms the would produce alternative fuels.
He was listed on Time Magazine's 100 list of the most influential people in both 2007and 2008
http://www.dailymail.co.uk/sciencetech/article-1279988/Artificial-life-created-Craig-Venter--wipe-humanity.html
